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He completed graduate work at the University of Pittsburgh, in 1963, followed by three years of post-graduate studies at Yale. (b) denaturation → extension → annealing There chapter wise Test papers with complete solutions are available for download in myCBSEguide website and mobile app. (d) assimilator. (a) Restriction endonuclease (b) the name of strain (d) Thermus aquaticus (b) making cells impermeable for DNA It has applications in various sectors such as medicine and agriculture. (d) Availability of ‘thermostable’ DNA polymerase (b) size only, Question 33. Which of the following is required to perform polymerase chain reaction ? biotechnology : principles and processes INTRODUCTION Biotechnology may be defined as the use of micro-organism, animals, or plant cells or their products to generate different products at industrial scale and services useful to human beings. While isolating DNA from bacteria, which of the following enzymes is not used ? 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